WHAT IS ICSI ? ICSI (Intra cytoplasmic sperm Injection) is the best method of fertilization. It is the process of injecting the selected best quality sperm in to the mature egg. ICSI Process involves usage of specialized manipulators and microscope for pickup of selected sperms. The hollow needle is used to inject the selected sperms in to the inner part of the egg (Cytoplasm). Approximately 70 -85% of eggs injected with best quality sperms show evidence of fertilization which is really good. PATIENTS SELECTION – WHO REQUIRES ICSI? This procedure has been considered a breakthrough in the management of patients with below mentioned conditions. Men with low sperm count / poor motility. Increased number of abnormal sperms. Previously failed IVF CYCLE. Azoospermia. TESA Retrieved sperms – Testicular aspiration of sperms. For females where total number of eggs retrieved is less than 5. ICSI PROCEDURE It requires specialized ICSI Microscope and special holding pipette. ICSI needles to pick up the sperms and immobilize it. The ICSI needle is carefully introduced in to cytoplasm of the egg and the sperm is injected in to the egg and the needle gently removed. The eggs are reviewed for growth 18-20 hours later for evidence of fertilization. IVF/ ICSI – THE PROCESS DAY TO DAY SUPER OVULATION In assisted reproduction, chances of conception can be increased by stimulating the ovaries to produce required number of eggs. This is termed as super ovulation and can be brought about by using drugs (Gonadotrophins) which mimic the action of the body’s natural hormones. Drug doses and combinations are calculated for each individual depending on a number of factors Including age, medical history, body mass index, antral follicle count (number of follicles on Day 2) and hormone values. Details will be given to each patient in the form of an individual "Protocol" letter by the medical staff.Best Fertility Care Centre, Advanced Fertility Care Centre, Ashwini Hospital Moondrumavadi K.Pudur Madurai -07 .Maternal & Child Health Care.